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KMID : 0380619800120020097
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Korean Journal of Food Science and Technology
1980 Volume.12 No. 2 p.97 ~ p.102
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Production of Fungal Lipids Ⅰ. On Intracellular Fungal Lipids
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신동화/Shin, Dong Hwa
서기봉/민병용/유진영/임호/Suh, Kee Bong/Min, Byong Yong/Yoo, Jin Young/Yim, Ho
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Abstract
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Cladosporium fulvum, Aspergillus ochraceus, Aspergillus terreus and N-1 (unidentified species) were cultured on the artificial media containing sucrose as a carbon source at 20, 25 and 30℃ for 10 to 12 days. The lipids in the felts were extracted with chloroform-methanol mixture and the class composition and fatty acids of the lipid were determined. The summarized results are as follows
1. The average felts produced by each species per 100 ㎖ of media were 3.82±0.30 g for Cl. fulvum, 2.62±0.23 g for Asp. ochraceus, 4.24±0.25 g for Asp. terreus and 4.62±0.10 g for N-1. Their crude fat contents 27.5±1.61 %, 50.47±1.00 %, 46.6±1.59 % and 33.78 % and the fat coefficient 6.92, 8.88, 13.01 and 10.28, respectively.
2. The lipids produced by these species were mainly composed of triglyceride and the next free fatty acid in Cl. fulvum and N-1 and phospholipid Asp. ochraceus and Asp. terreus.
3. The major fatty acids of the lipids were in order of oleic, palmitic, linoleic and stearic acids in Asp. ochraceus, Asp. terreus and Cl. fulvum and linoleic, palmitic, oleic and stearic acid in N-1. The total percentage contents of these major fatty acids were over 98 % the former and over 95 % the latter.
4. The constituent fatty acids of the lipid were changed depending on the incubation temperature but hardly found a certain tendency except linoleic acid which was higher at lower temperature.
5. The total percentages of unsaturated fatty acids in the lipids were 50∼60 % and comparatively higher at lower incubation temperature.
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